Coupling of groundwater, river level and rainfall in an upland floodplain

Upland floodplains provide an important function in regulating river flows and controlling the coupling of hillslope runoff with rivers. To investigate the responses of floodplain groundwater to river flows and rainfall events, a small floodplain in an upland area of the River Tweed catchment, Scotland, was characterised using geophysics, 3D geological mapping and hydrogeological testing; and monitoring undertaken from September 2011 to February 2013 of: groundwater levels in five pairs of piezometers; river stage and flow at the upstream and downstream limits of the study site; soil moisture on the adjacent hillslope; and meteorological parameters. Periodical groundwater chemistry and residence data were also collected. The floodplain aquifer is permeable throughout but partially stratified, comprising dominantly alluvial and glaciofluvial sandy gravels between 8 and 15m interspersed with thin, intermittent layers of low permeability silts, clays and peats. Overlying the gravel aquifer is a partial thin cover of low permeability alluvial silts, and it is underlain dominantly by low permeability glaciolacustrine silts and clays. High permeability solifluction deposits mantle much of the adjacent hillslope and provide a rapid connection to the floodplain aquifer. The unusually wet year of 2012 provides a good example of how a temperate upland floodplain responds to consistently high rainfall. Statistical analysis and graphical interpretation of groundwater level, rainfall, soil moisture and river stage demonstrates that: 1) dominant groundwater flow within the floodplain is in the same direction as the river, from up-valley to down-valley; 2) soil moisture in the hillslope is strongly correlated with local rainfall, but groundwater across much of the floodplain is more strongly influenced by river stage; except 3) groundwater near the edge of floodplain, which responds more slowly to local rainfall and river stage changes ; and 4) subsurface flow from the hillslope to the floodplain occurs during high rainfall events. A detailed investigation of three flood events, when the river rose above bank level and flooded adjacent fields and groundwater became artesian in parts of the floodplain, suggests that antecedent moisture conditions can partly explain the differences in groundwater response during different flood events, where high intensity or long duration rainfall can cause saturated soil conditions, reducing soil water storage capacity and hence promoting flood conditions. A conceptual model based on field data of groundwater flow after storm events during antecedent unsaturated and saturated soil conditions is presented

Factors associated with parents’ experiences using a knowledge translation tool for vaccination pain management: a qualitative study

Background: Vaccination is a common painful procedure for children. Parents’ concern regarding vaccination pain is a significant driver of vaccine hesitancy. Despite the wealth of evidence-based practices available for managing vaccination pain, parents lack knowledge of, and access to, these strategies. Knowledge translation (KT) tools can communicate evidence-based information to parents, however little is known about what factors influence parents’ use of these tools. A two-page, electronic KT tool on psychological, physical, and pharmacological vaccination pain management strategies for children, was shared with parents as part of a larger mixed methods study, using explanatory sequential design, exploring factors related to uptake of this KT tool. The aim of this qualitative study was to understand what influenced parents’ perceptions of the relevance of the KT tool, as well as their decision as to whether to use the tool. Methods: A qualitative descriptive design was used. A total of 20 parents of children aged 0–17 years (n = 19 mothers) reviewed the KT tool ahead of their child’s upcoming vaccination and participated in a semi-structured interview at follow-up. Interviews were recorded, transcribed verbatim, and analyzed with reflexive thematic analysis using an inductive approach. Results: The analysis generated three interrelated themes which described factors related to parents’ use of the KT tool: (1) Relevance to parents’ needs and circumstances surrounding their child’s vaccination; (2) Alignment with parents’ personal values around, and experiences with, vaccination pain management (e.g., the importance of managing pain); and (3) Support from the clinical environment for implementing evidence-based strategies (e.g., physical clinical environment and quality of interactions with the health care provider). Conclusions: Several factors were identified as central to parents’ use of the KT tool, including the information itself and the clinical environment. When the tool was perceived as relevant, aligned with parents’ values, and was supported by health care providers, parents were more inclined to use the KT tool to manage their children’s vaccination pain. Future research could explore other factors related to promoting engagement and uptake when creating parent-directed KT tools for a range of health-related contexts

Amino Acids Are an Ineffective Fertilizer for Dunaliella spp. Growth

Autotrophic microalgae are a promising bioproducts platform. However, the fundamental requirements these organisms have for nitrogen fertilizer severely limit the impact and scale of their cultivation. As an alternative to inorganic fertilizers, we investigated the possibility of using amino acids from deconstructed biomass as a nitrogen source in the genus Dunaliella. We found that only four amino acids (glutamine, histidine, cysteine, and tryptophan) rescue Dunaliella spp. growth in nitrogen depleted media, and that supplementation of these amino acids altered the metabolic profile of Dunaliella cells. Our investigations revealed that histidine is transported across the cell membrane, and that glutamine and cysteine are not transported. Rather, glutamine, cysteine, and tryptophan are degraded in solution by a set of oxidative chemical reactions, releasing ammonium that in turn supports growth. Utilization of biomass-derived amino acids is therefore not a suitable option unless additional amino acid nitrogen uptake is enabled through genetic modifications of these algae

Effect of vitamin D supplementation on blood pressure:a systematic review and meta-analysis incorporating individual patient data

D-PRESSURE Collaboration: et al.[Importance]: Low levels of vitamin D are associated with elevated blood pressure (BP) and future cardiovascular events. Whether vitamin D supplementation reduces BP and which patient characteristics predict a response remain unclear.[Objective]: To systematically review whether supplementation with vitamin D or its analogues reduce BP.[Data Sources]: We searched MEDLINE, CINAHL, EMBASE, Cochrane Central Register of Controlled Trials, and http://www.ClinicalTrials.com augmented by a hand search of references from the included articles and previous reviews. Google was searched for gray literature (ie, material not published in recognized scientific journals). No language restrictions were applied. The search period spanned January 1, 1966, through March 31, 2014.[Study Selection]: We included randomized placebo-controlled clinical trials that used vitamin D supplementation for a minimum of 4 weeks for any indication and reported BP data. Studies were included if they used active or inactive forms of vitamin D or vitamin D analogues. Cointerventions were permitted if identical in all treatment arms.[Data Extraction and Synthesis]: We extracted data on baseline demographics, 25-hydroxyvitamin D levels, systolic and diastolic BP (SBP and DBP), and change in BP from baseline to the final follow-up. Individual patient data on age, sex, medication use, diabetes mellitus, baseline and follow-up BP, and 25-hydroxyvitamin D levels were requested from the authors of the included studies. For trial-level data, between-group differences in BP change were combined in a random-effects model. For individual patient data, between-group differences in BP at the final follow up, adjusted for baseline BP, were calculated before combining in a random-effects model.[Main Outcomes and Measures]: Difference in SBP and DBP measured in an office setting.[Results]: We included 46 trials (4541 participants) in the trial-level meta-analysis. Individual patient data were obtained for 27 trials (3092 participants). At the trial level, no effect of vitamin D supplementation was seen on SBP (effect size, 0.0 [95% CI, −0.8 to 0.8] mm Hg; P = .97; I2 = 21%) or DBP (effect size, −0.1 [95% CI, −0.6 to 0.5] mm Hg; P = .84; I2 = 20%). Similar results were found analyzing individual patient data for SBP (effect size, −0.5 [95% CI, −1.3 to 0.4] mm Hg; P = .27; I2 = 0%) and DBP (effect size, 0.2 [95% CI, −0.3 to 0.7] mm Hg; P = .38; I2 = 0%). Subgroup analysis did not reveal any baseline factor predictive of a better response to therapy.[Conclusions and Relevance]: Vitamin D supplementation is ineffective as an agent for lowering BP and thus should not be used as an antihypertensive agent.Peer reviewe

Fast FIB-milled Electron-transparent Microchips for in situ TEM Investigations

In this work we present a fast approach to 50 nm resolution structures defined in a generic TEM-chip template in few minutes. While creating complex electrical and NEMS circuits for a specific insitu TEM experiment can be a cumbersome process, microchips with 100 nm thin flakes of single crystalline silicon and silicon nitride membrane templates suspended from the edge, can be patterned in less than 15 minutes using focused ion beam milling. This approach allows a FIB-SEM user to create free-form NEMS structures for nanoresonators, actuators, heaters, resistors or other structures for insitu TEM devices or materials research using the same template. We demonstrate insitu environmental TEM analysis of Au film migration on silicon during resistive heating of a microbridge, and show how the conductance of focused ion beam milled single crystalline silicon nanowires can be adjusted insitu over two decades using a high current to recrystallise the structure

Natural HLA Class I Polymorphism Controls the Pathway of Antigen Presentation and Susceptibility to Viral Evasion

HLA class I polymorphism creates diversity in epitope specificity and T cell repertoire. We show that HLA polymorphism also controls the choice of Ag presentation pathway. A single amino acid polymorphism that distinguishes HLA-B*4402 (Asp116) from B*4405 (Tyr116) permits B*4405 to constitutively acquire peptides without any detectable incorporation into the transporter associated with Ag presentation (TAP)-associated peptide loading complex even under conditions of extreme peptide starvation. This mode of peptide capture is less susceptible to viral interference than the conventional loading pathway used by HLA-B*4402 that involves assembly of class I molecules within the peptide loading complex. Thus, B*4402 and B*4405 are at opposite extremes of a natural spectrum in HLA class I dependence on the PLC for Ag presentation. These findings unveil a new layer of MHC polymorphism that affects the generic pathway of Ag loading, revealing an unsuspected evolutionary trade-off in selection for optimal HLA class I loading versus effective pathogen evasion

Susceptible host availability modulates climate effects on dengue dynamics

Experiments and models suggest that climate affects mosquito-borne disease transmission. However, disease transmission involves complex nonlinear interactions between climate and population dynamics, which makes detecting climate drivers at the population level challenging. By analysing incidence data, estimated susceptible population size, and climate data with methods based on nonlinear time series analysis (collectively referred to as empirical dynamic modelling), we identified drivers and their interactive effects on dengue dynamics in San Juan, Puerto Rico. Climatic forcing arose only when susceptible availability was high: temperature and rainfall had net positive and negative effects respectively. By capturing mechanistic, nonlinear and context-dependent effects of population susceptibility, temperature and rainfall on dengue transmission empirically, our model improves forecast skill over recent, state-of-the-art models for dengue incidence. Together, these results provide empirical evidence that the interdependence of host population susceptibility and climate drives dengue dynamics in a nonlinear and complex, yet predictable way.R35GM133439 - NIH HHS; DBI-1667584 - National Science Foundation; DEB-1655203 - National Science Foundation; 00028335 - Lenfest Foundation; Stanford University: Bing Fellowship in Honor of Paul Ehrlich, Stanford Data Science Scholars program, Lindsay Family E-IPER Fellowship, Illich-Sadowsky Interdisciplinary Graduate Fellowship, Terman Fellowship, King Center for Global Development seed grant; SERDP 15 RC-2509 - U.S. Department of Defense; University of California San Diego: McQuown Chair in Natural Sciences; DBI-1611767 - National Science Foundation; RAPID DEB-1640780 - National Science Foundation; R35GM133439 - NIH HHS; Hellman Foundation: Hellman Faculty Fellowship; Stanford Woods Institute for the Environment: Environmental Ventures Program; DEB-1518681 - National Science Foundation; R35 GM133439 - NIGMS NIH HHS; DEB-2011147 - National Science Foundationhttps://www.biorxiv.org/content/biorxiv/early/2020/10/19/2019.12.20.883363.full.pdfAccepted manuscrip